One Step Synthetic Cannabis (K2) Test has set the screen cut-off for positive specimens at 25 ng/mL for JWH-018 Pentanoic Acid and JWH-073 Butanoic Acid as the calibrators. The test device has been proved to detect above the cut-off level of the target drugs in urine at 5 minutes.
B.Specificity and cross reactivity
To test the specificity of the test, the test device was used to test Synthetic Cannabis (K2), its metabolites and other components of the same class that are likely to be present in urine, All the components were added to drug-free normal human urine. These concentrations below also represent the limits of detection for the specified drugs or metabolites.
JWH-018 Pentanoic Acid
JWH-073 Butanoic Acid
JWH-018 (Spice Cannabinoid)
JWH-018 4-Hydroxypentyl metabolite-D5 (indole-D5)
JWH-073 (Spice Cannabinoid)
JWH-073 3-Hydroxybutyl metabolite
JWH-073 3-Hydroxybutyl metabolite-D5 (indole-D5)
JWH-210 5-Hydroxypentyl metabolite
AM2201 4-Hydroxypentyl metabolite
The assay is intended to verify an intoxication in patients. It provides a qualitative, preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary results are positive.
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