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- Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay. - Take out the test card from the foil pouch and place it horizontally. - Place 10μL of the prepared specimen into the sample hole, matching the window CHW. Then drop 3 drops (approx. 100μL) of assay buffer CHW into the sample hole. Start the timer. - Collect 20μL of the prepared specimen into a vial of EHR-ANA assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole “S” of the test card, respectfully matching the windows EHR, ANA. Start the timer. - Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
INTENDED USE
Ehrlichia - Anaplasma - Heartworm Combo Test is a test cassette to diagnose the presence of tick-borne diseases antibodies against Ehrlichia canis, Ehrlichia ewingii, Anaplasma phagocytophilum and Anaplasma platys, and Dirofilaria immitis in dog’s serum or plasma specimen. Assay Time: 5-10 minutes Sample: Serum, plasma, or whole blood.
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- Collect 20μL of the prepared specimen into a vial of EHR-ANA assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into the sample hole “S” of the test card, respectfully matching the windows EHR, ANA. Start the timer. - Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid. 
